Kaixin micro test ESD18VB03-0201
EL-C1600N100013-B
微盟ME2188A33PG
Test - lowercase jpg
(a) Collection of clinical samples
A, blood sample
Some physiological factors, such as smoking, eating, exercise, mood swings, pregnancy, body position, etc., can affect changes in certain components of the blood, and some even have day and night changes. Therefore, the collection of blood specimens should avoid physiological factors as much as possible, and it is advisable to agree on conditions. If it is unavoidable, the factor should be indicated on the specimen.
1. Peripheral blood
Generally, the inner side of the ring finger of the left hand is selected for blood collection, and the site should be free of frostbite, inflammation, edema, and damage. If the part does not meet the requirements, replace it with other finger parts. For burn patients, blood can be collected from the intact skin. Since some blood routine tests such as white blood cell count, classification, etc. are excessively affected by physiological factors, the conditions should be as uniform as possible. For the detection of in vivo and coagulation tests (such as platelet count, bleeding time or clotting time), it is important to pay attention to whether the patient has used anticoagulant or procoagulant drugs in order to reduce or avoid the influence of interference factors.
2. Venous blood
In addition to anti-coagulation venous blood plasma, which is involved in various hemostasis and thrombus testing projects, most of the current testing items can be directly used for venous blood serum. In the serum testing program, some (such as blood sugar, blood lipids, etc.) are greatly affected by diet and day and night factors, generally in the early morning fasting blood specimens; some in the blood decay faster (serum enzyme activity such as ACP activity, etc.), The storage activity of 0~4°C is weakened. The detection of these items must be timely and rapid; some (such as creatine kinase) are greatly affected by factors such as exercise. It is also important to avoid the occurrence of hemolysis during blood draw, especially in the determination of serum potassium, LDH, etc.
B, urine sample
Like blood samples, urine specimens are also affected by factors such as diet, exercise, and drug dosage, especially the effects of diet, so morning urine is generally better than random urine. Morning urine refers to the first urine specimen after getting up in the morning. It is concentrated and acidified, and the formation points (such as blood cells, epithelial cells, and tubular shape) are relatively concentrated for easy observation. Random urine is a random urine, easy to stay, but is more affected by diet, exercise, drugs, prone to false positive and false negative results, such as dietary proteinuria, dietary diabetes, vitamin C interference occult blood results. Postprandial urine (patient urine collected 2 hours after lunch) is suitable for urine sugar, urine protein and urobilinogen examination. At this time, urine specimens can increase the sensitivity of the test and detect milder lesions. The 12-hour urinary cell count, which is the Addis count (all urine left after the emptying of the bladder at 8 pm to 8:00 am the next day, due to the longer time, the bacteria are easy to breed, must be added with preservative formaldehyde. 24-hour urine first Quantification of chemical substances in all urine) after 8 hours of emptying of the bladder at 8 am, including protein, sugar, urine 17-ketone, 17-hydroxysteroid, catecholamine, Ca2+, etc. Substance, choose different preservatives for preservation. Clean mid-stage urine is mostly used for urinary bacterial culture. It requires sterility and samples are taken after rinsing the vulva.
All urine specimens should be collected in a sufficient amount, at least 12 ml, preferably 50 ml. All urine must be collected at regular intervals. For female patients, vaginal secretions, blood-contaminated urine specimens should be avoided.
C, stool sample
The detection of stool specimens has important reference value for judging diseases of the digestive system. At the time of collection, it is required to use a clean bamboo stick to select feces containing abnormal components such as mucus and pus, and the feces without abnormal appearance must be taken from the surface, deep and feces. The count of parasites and eggs should be collected for 24 hours of feces. Check the dysentery amoeba trophozoite should be checked immediately after defecation, from the pus and soft place, heat preservation for inspection. When examining the eggs of Schistosoma japonicum, the mucus and pus and blood should be taken. At least 30 g of feces should be taken when hatching the mites, and it should be treated as soon as possible. Check the mites eggs with a clear film swab and swab from the wrinkles around the anus at 12 pm or early morning defecation and immediately microscopically. The occult blood test (chemical method), fasting meat and animal blood foods on the 3rd day before the test and banned iron, vitamin C and so on. All fecal specimens should be inspected within 1 hour after collection to prevent the formation of digestive enzymes and pH damage. If it is too late to detect, it should be buffered with PBS in time and centrifuged to remove the supernatant.
D, cerebrospinal fluid sample
The cerebrospinal fluid specimens are sent for inspection immediately after collection. If they are placed for too long, the test results will be affected: if the cells are denatured and destroyed, the counting and classification will be inaccurate; some chemical substances such as glucose will reduce the decomposition content; the bacteria will be autolyzed to affect the detection rate of bacteria. . After the cerebrospinal fluid is extracted, three sterile tubes are generally divided. The first tube is used for bacterial culture, the second tube is used for chemical analysis and immunological examination, and the third tube is used for general traits and microscopic examination. The order of the three tubes should not be reversed. Due to the difficulty in collecting specimens, all inspection and testing procedures should be safe.
E, chest and ascites samples
As with the cerebrospinal fluid specimens, the collected specimens are safe and promptly sent for inspection. Generally, three tubes are also dispensed, one tube for routine cytological examination, one tube for biochemical examination, and one tube for bacterial culture, and the order is the same as cerebrospinal fluid.
F, prostatic fluid sample
The specimen of prostatic fluid is collected from the prostate after massage. When the amount of fluid is small, it is directly dripped on the slide and sent for inspection. It is necessary to prevent the specimen from being evaporated and dried. When the amount is large, it is collected in a clean and dry test tube. If the prostatic fluid is not massaged, the urine sediment after the massage can be checked.
G, semen sample
Semen specimens should be forbidden for 3 to 7 days before collection. After the urine is drained, the semen can be directly discharged into a clean container by masturbation or other methods, and kept warm and promptly sent for inspection. Due to the large variation in sperm production during the day, it should be checked 2 to 3 times (1 to 2 weeks apart for diagnosis).
H, vaginal secretion samples
The vaginal specimens should be banned from sexual intercourse, bathing, vaginal examination, vaginal lavage and topical medicine 24 hours before collection. The equipment used for the preparation should be cleaned. Generally, a cotton swab soaked with salt water is taken from the deep part of the vagina or the back of the vagina, the cervical canal, etc.
Observation of vaginal secretions after saline smear, menstrual women should not be examined
Check the vaginal secretions specimens.
(II) Sample preparation for ELISA
There must be a complete plan before collecting the sample, and it must be clear whether the ingredients to be tested are sufficiently stable. Samples that were tested on the day after collection were stored in time at 4 °C for later use. For samples that are retested every other day, they should be stored at -20 °C in a timely manner after sub-packaging. If conditions permit, it is best to freeze at -70 °C for later use. Specimens should avoid repeated freezing and thawing. Liquid specimens: including serum, plasma, urine, pleural and ascites, cerebrospinal fluid, cell culture supernatant, etc.
1. serum:
The blood is naturally solidified at room temperature for 10-20 minutes, and then centrifuged for about 20 minutes. Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
2. plasma:
EDTA, sodium citrate or heparin should be selected as an anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
3. Urine:
Collect with a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. The chest and ascites and cerebrospinal fluid are implemented with reference to this.
4. Cell culture supernatant:
When detecting secreted components, collect them in a sterile tube. Centrifuge for about 20 minutes - 3000 rpm). Collect the supernatant carefully.
5. Cultured cells
When measuring the components in the cells, the cell suspension was diluted with PBS (pH 7.2. 7-7), and the cell concentration reached about 1 million/ml. By repeatedly freezing and thawing or adding tissue protein extraction reagents, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
6. Tissue specimen
After cutting the specimen, weigh the weight. Add a certain amount of PBS, pH 7. 4 . It was quickly frozen and stored in liquid nitrogen for use. The specimen still maintains a temperature of 2-8 ° C after melting. Add a certain amount of PBS (pH 7.4), or tissue protein extraction reagent, and homogenize the specimen by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. One part of the package is to be tested, and the rest is frozen for use.
We provide free testing services: use our Elisa kit, only charge for the kit, all other auxiliary reagents are free. Based on the experimental workload and difficulty level, we will agree on the experimental cycle, complete the entrusted experiment with quality and quantity, and send the results and materials to you in free.
(3) ELISA test generation cycle content, etc. Service number service item Description of the submission result cycle RGSc1 Indirect ELISA test customer provides test material test report 1 week RGSc2 Sandwich ELISA test customer provides test material test report 1 week and test reagent Box RGSc3 competition method ELISA test customer provides test material test report 2 weeks and the test kit should be marked with the following conditions: specimen number; 2, measured items; 3, whether to do duplicate holes; 3, contact information; 4 Whether the specimen is sent back after the experiment.
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