Determination of Total Phenol Content in Water by UV-1200 Ultraviolet Spectrophotometry - Master's thesis - Dissertation

Key words: ultraviolet spectrophotometry; total phenol; US analytical instrument ; UV-1100; UV-1200 phenol is a harmful substance in industrial wastewater, if it flows into the river, the water quality will be affected Contamination, therefore, when testing the hygienic quality of drinking water, the phenol content in the water needs to be determined. First, the purpose of the experiment 1, master the principle and method of measuring phenol by ultraviolet spectrophotometry. 2. Familiar with the basic operation technology of the ultraviolet spectrophotometer. 2. Principle of the method Compounds with benzene ring structure have strong characteristic absorption peaks in the ultraviolet region, and the first type of substituents (activating groups) on the benzene ring make absorption stronger, while phenol has 270 nm at 270 nm. Characteristic absorption peak, the degree of absorption is proportional to the content of phenol, so the content of total phenol in water can be directly determined by ultraviolet spectrophotometry according to Lambert-Beer's law. Third, the instrument and reagent 1, the US analysis of UV spectrophotometer UV-1100; UV-1200; 2, phenol standard solution, 250mg / L: accurately weigh 0.0250g of phenol in a 250mL beaker, add deionized water 20mL to dissolve, Transfer to a 100 mL volumetric flask, dilute to the mark with deionized water, and shake well. 4. Contents and steps 1. Configuration of standard series solution Take 5 25ml colorimetric tubes, add 1.00, 2.00, 3.00, 4.00, 5.00mL phenol standard solution, dilute with deionized water to the mark and shake for testing. 2. Measurement of absorption curve Take any solution in the above-mentioned standard series of solutions, and use a 1 cm quartz cuvette with a solvent blank as a reference. The absorbance is measured every 5 nm in the wavelength range of 220-350 nm. 3. Measurement of the standard curve The absorbance of the standard series solution was measured at a maximum absorption wavelength (λmax) of phenol using a 1 cm quartz cuvette with a solvent blank as a reference. 4. Measurement and measurement of water sample Under the same conditions of the standard solution, the absorbance of the water sample is measured. 5. Data recording and processing 1. The absorbance value of the same standard solution at different wavelengths is recorded. The absorbance is plotted on the ordinate and the wavelength is horizontal. The coordinates are plotted to absorb the curve, find λmax, and calculate its max. 2, the list records the absorbance of the solution and the water sample, the absorbance is the ordinate, the concentration of the standard series solution is the ordinate, and the curve is drawn; the concentration of the standard solution is calculated according to the absorbance of the water sample and the water is calculated. The content of phenol (g/L) in the sample. Key words: ultraviolet spectrophotometry; total phenol; aesthetic instrument ; UV-1100; UV-1200