ELISA kit experiments have no absorbance or low absorbance value how to break? - Database & Sql Blog Articles

Photocoupler

In the process of doing ELISA experiments, it is inevitable that there will be a problem. When the problem occurs, we need to find the cause of the problem, and then come up with the corresponding solution according to the reason! In today's article, we will analyze for everyone: When doing the ELISA experiment, after adding the reaction stop solution, how can it be broken if there is no absorbance or low absorbance?

After the reaction stop solution was added, there was no absorbance or the absorbance was low.

a. Cause: No enzyme label was added.

Solution: Please follow the steps.

b. Reason: The contents of the kit did not fully recover.

Solution: Make sure the contents of the kit are warmed up before proceeding.

c. Reason: The room temperature is too low.

Solution: If the room temperature is too low (<19 ° C), please extend the incubation time appropriately in step 4.

d. Cause: The cleaning solution of the kit was not used for cleaning.

Solution: Please wash with the cleaning solution provided in the kit.

e. Reason: The kit has expired.

Solution: Replace the kit with the expiration date.

If you have any technical problems during the experiment, you can call us for technical consultation. Shanghai Jinma has one-to-one full-course guidance service, high-quality ELISA test kit, so that you win the first step. The company's main: elisa kit, human Elisa kit, rat Elisa kit, mouse Elisa kit, biological reagents, serum, antibodies, media, standards, domestic serum, GIBCO fetal bovine serum.